These compounds are widespread in the plant kingdom as part of our daily diet and are attractive as natural antioxidants. Antioxidant assay the antioxidant activity of the plant extracts was tested using two methods. In vitro assays for the free radical scavenging capacity are usually based on the inactivation of radicals, such as hydroxyl oh and nitric oxide no radicals. Previous studies have shown that ut exhibited antioxidant activity even at low concentrations 9,22,23. Pdf antioxidant activity by dpph radical scavenging. Antioxidantrich extracts of terminalia ferdinandiana. The antioxidants present in the plant extracts react with oxidizing agents and their potential to reduce the oxidizing agents was evaluated spectrophotometrically 30. Evaluation of the in vitro and in vivo antioxidant.
This is a typical problem in studies using plant extracts. The determination of the total antioxidant activity frap assay in the extract is a modified method of benzie and strain 30. Total phenolics content of leaf extract also highest 245. The ftc method was used to measure the amount of peroxide at the beginning of the lipid peroxidation, in which peroxide reacts with ferrous chloride and form ferric ion. The present study evaluated the antioxidant effects of methanolic seed extract of a. Shanab2 1biochemistry department, faculty of agriculture, cairo university, giza, egypt. In vitro antioxidant activity of rubus ellipticus fruits. Variations in plant material, extraction method, processing and antioxidant assays employed might affect the concentrations of active compounds that could be reflected in the antioxidant activity. The antioxidant assay of various extracts, using 2, 2diphenyl1picrylhydrazyl radical scavenging model, revealed that the ethanol and nbutanol extracts of d. Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. Comparative analysis of the antioxidant activity of cassia. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. The reacted solution obtained 1 ml was used for tba assay. Phytochemical screening and evaluation of antioxidant and.
In the first thirtyfour pages, a total of three hundred and forty articles appeared and. The powder samples and methanol extract of 11 medicinal plants were subjected to analysis of proximate composition and measurement of antioxidant activity. Screening of in vitro antioxidant activity of methanolic. Reducing power assay the reducing power of the extract was evaluated according to oyaizu method24. Abts radical cation was produced in the stable form using potassium persulphate. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. Original article comparison of abts, dpph, frap, and orac. The antioxidant activity of plant extracts was determined by different in vitro methods such as the dpph free radical scavenging assay and reducing power methods. Therefore, the assay for screening germplasm and hybrids should be.
Methanolic extracts of cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. Research article open access antioxidant activity, total. Antioxidant properties of extracts from selected plant materials. Antioxidant and antimicrobial properties of five medicinal. Antioxidant activity of methanol extracts of different. The number of methods and variations in methods to measure antioxidants in botanicals that have been proposed has also increased considerably. The results showed that all the plant parts possessed antioxidant properties. Quantification of the antioxidant activity of plant.
Comparison of dpph and abts assays for determining. Antioxidant activity of methanol extracts of different parts of lantana. The antioxidant activity was investigated using the ferric reducing antioxidant power frap assay for plant extracts was investigated according to the method of oyaizu 1986 27. Antioxidant activities measured in methanol extract obtained using abts, dpph, frap, and orac assays from a single extract were measured three times to test the reproducibility of the assays. The current phytochemical study was carried out on the most potent extract in cellfree antioxidant assays. Different parameters studied include phenolic contents, moisture, ash, crude fiber, fats and waxes. Comparison of dpph and abts assays for determining antioxidant potential of water and methanol extracts of spirulina platensis emad a. Preparation of plant extracts for antioxidant property analysis and total polyphenol content. Leaf disc assays for rapid measurement of antioxidant. Dpph assay revealed that leaf extract had the highest antioxidant activity comparable with vitamin c leaf ic 50 16. Their extracts were analyzed using the following methods. The results from the antioxidant assay showed that extract of all plants can scavenge the radical to a certain extent.
Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. Phytochemicals, antioxidant and antimicrobial potentials. Pdf evaluation of antioxidant activity of medicinal plant extracts. Evaluation of antioxidant activity of medicinal plant. Antioxidant properties of several medicinal plants growing. Evaluation of antioxidant activity of medicinal plant extracts.
Dpph assay revealed that leaf extract had the highest antioxidant activity. Antioxidant activity by dpph assay of potential solutions. It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds cook and samman, 1996. Pdf methods for determining the antioxidant activity. Comparative study of antioxidant properties and total. Phytochemical screening and antioxidant activity of moroccan. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase. Medicinal plants used in the traditional medicine are wellknown significant sources of natural antioxidants. The different extracts were dissolved in ethanol at a concentration of 50200 mgml.
Ic50 which is an inhibitory concentration of each extract required to reduce 50% of the nitric oxide. Dpph, ferric reducing power and nitric oxide as an antioxidant model. Osborne 2, 1 queensland alliance for agriculture and food innovation qaafi, the university of queensland, health. Antioxidant capacity of selected plant extracts and their. The content of total phenolics in the extracts was determined spectrometrically according to the folin. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate.
Comparison of abts, dpph, frap, and orac assays for. One gram of dry plant material was homogenized with 20 ml of solvent solution acetoneultrapure waterglacial acetic acid, 70. In this assay, a molecule or antioxidant with weak ah bonding will react with a stable free radical dpph 2,2diphenyl1picrylhydrazyl. Fruit, vegetable and plant extractions can be done using acidmethanol for e. The first step to quantify the antioxidant activity of a plant extract is to select the right method. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. To obtain the extracts the methodology previously described by michiels et al. For in vitro dpph, abts, and ppr antioxidant assays, 10. Inhibition data percentage inhibition were linearized against the concentrations of each extract and standard antioxidant. Pdf different extracts from five medicinal plants in terms of phenolic compounds and.
Ferric reducing antioxidant power colorimetric assay protocol. Evaluation of antioxidant capacity of plant extracts by. Dpph free radical scavenging activity of the extracts of. However, use of bpe in antioxidant assays has shortcomings in that 1 bpe has lottolot variability in reactivity to peroxyl radicals, leading to inconsistency in assay results 15. Issn total antioxidant capacity tac of fresh leaves of. The antioxidant activity of the aerial part extract of m. Evaluation of antioxidant activity and total phenol in. Total phenolic, total flavonoid, tannin content, and. The dpph and frap assays showed no differences among determinations, while the abts and orac assays differed among runs table 2. Antioxidant properties of the methanol extracts of the. Antioxidant activity of plant extracts containing phenolic. A variety of fruit, vegetable and plant samples, beverages as well as serum and plasma can be used with this assay. Aqueous extracts of 30 plants were investigated for their antioxidant properties using dpph and abts radical scavenging capacity assay, oxygen radical absorbance capacity orac assay, superoxide dismutase sod assay, and ferric reducing antioxidant potential frap assay.
Assays assessing nonenzymatic hydrogen peroxide antioxidant capacities are often hampered by the high uv absorption of the sample itself. L with nanopure water or methanol in 96well plate, and. Antioxidant activity was assessed by using 2,2diphenyl1picrylhydrazyl dpph assay, reducing power activity, 2,2azinobis3. The antioxidant potential of some herbal plant extracts commercial products was measured using various in vitro assays. Reviews of some of the methods have been published recently. Unlike hydrophilic antioxidants, antioxidant assays available for hydrophobic compounds are limited. Antioxidant and in vitro cytotoxic activity of extracts of. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural systems, plant biotechnology and. Review on in vivo and in vitro methods evaluation of. Pdf the antioxidant potential of some herbal plant extracts commercial products was measured using various in vitro assays. As opposed to frap method the flowers had greater antioxidant activity as leaves. Among the extracts from curcuma longa, caffea arabica, tribulus terrestris, bacopa monnieri and trigonella foenum graecum, the curcuma longa and coffee bean extract. Over the years, research on antioxidants and medicinal plants has gained enormous popularity and emerged as a potential therapeutic to prevent free radical generated damage in the human body. The assays employed were ferric reducing antioxidant power, trolox equivalent antioxidant.
Total phenolic content was also determined by the folin. Medicinal plants derived natural antioxidants, which are in the form of raw extracts andor chemical constituents, are very efficient to block the process of oxidation by neutralizing free radicals 3. In vitro antioxidant and anticancer activity of leea. Plants have a large number of bioactive compounds with high antioxidant activity.
There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to. Standardized methods for the determination of antioxidant. Antioxidant properties of the methanol extracts of the leaves and stems of celtis africana adeolu a. Evaluation of antioxidant activity of some plant extracts. Tocopherol was used as a standard to evaluate the antioxidative activity of samples. Several complementary methods have been proposed to assess the antioxidant activity of plant extracts and pure compounds. Plant material extracts were studied by in vitro methods, such as total phenolic. Which antioxidant assay is suitable for plant extract. Phenolics are the largest group of phytochemicals that account for most of the antioxidant activity in plants or plant products 14. Plant extract of 1, 10, 50 or 100 mg in 1 ml of ethanol was mixed with 2. Estimation of phytochemical content and antioxidant.
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