Antioxidant activity was assessed by using 2,2diphenyl1picrylhydrazyl dpph assay, reducing power activity, 2,2azinobis3. Standardized methods for the determination of antioxidant. Dpph, ferric reducing power and nitric oxide as an antioxidant model. Dpph assay revealed that leaf extract had the highest antioxidant activity. Antioxidant properties of several medicinal plants growing. Their extracts were analyzed using the following methods. Unlike hydrophilic antioxidants, antioxidant assays available for hydrophobic compounds are limited. Pdf antioxidant activity by dpph radical scavenging. Comparative analysis of the antioxidant activity of cassia. Discussion antioxidant capacity assays may be broadly classified as single electron transfer set and hydrogen atom transfer hat based assays. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. The content of total phenolics in the extracts was determined spectrometrically according to the folin. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to. Evaluation of antioxidant activity of medicinal plant.
Hydroethanolic extract of the aerial parts was fractionated using vacuumliquid. Medicinal plants derived natural antioxidants, which are in the form of raw extracts andor chemical constituents, are very efficient to block the process of oxidation by neutralizing free radicals 3. The current phytochemical study was carried out on the most potent extract in cellfree antioxidant assays. The antioxidant potential of some herbal plant extracts commercial products was measured using various in vitro assays. The antioxidant activity of plant extracts was determined by different in vitro methods such as the dpph free radical scavenging assay and reducing power methods. Antioxidant activity of plant extracts containing phenolic. Several complementary methods have been proposed to assess the antioxidant activity of plant extracts and pure compounds.
Which antioxidant assay is suitable for plant extract. Screening of in vitro antioxidant activity of methanolic. Evaluation of the in vitro and in vivo antioxidant. Comparison of abts, dpph, frap, and orac assays for. Antioxidant properties of the methanol extracts of the. As opposed to frap method the flowers had greater antioxidant activity as leaves. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. One gram of dry plant material was homogenized with 20 ml of solvent solution acetoneultrapure waterglacial acetic acid, 70. Evaluation of antioxidant activity of medicinal plant extracts. Total phenolic content was also determined by the folin. A variety of fruit, vegetable and plant samples, beverages as well as serum and plasma can be used with this assay.
Aqueous extracts of 30 plants were investigated for their antioxidant properties using dpph and abts radical scavenging capacity assay, oxygen radical absorbance capacity orac assay, superoxide dismutase sod assay, and ferric reducing antioxidant potential frap assay. Antioxidant properties of extracts from selected plant materials. Evaluation of antioxidant capacity of plant extracts by. Reducing power assay the reducing power of the extract was evaluated according to oyaizu method24. Preparation of plant extracts for antioxidant property analysis and total polyphenol content. Evaluation of antioxidant activity and total phenol in. To obtain the extracts the methodology previously described by michiels et al. Phytochemicals, antioxidant and antimicrobial potentials. Plant extract of 1, 10, 50 or 100 mg in 1 ml of ethanol was mixed with 2. Assays assessing nonenzymatic hydrogen peroxide antioxidant capacities are often hampered by the high uv absorption of the sample itself.
Phenolics are the largest group of phytochemicals that account for most of the antioxidant activity in plants or plant products 14. The antioxidant activity was investigated using the ferric reducing antioxidant power frap assay for plant extracts was investigated according to the method of oyaizu 1986 27. Quantification of the antioxidant activity of plant. Previous studies have shown that ut exhibited antioxidant activity even at low concentrations 9,22,23. The first step to quantify the antioxidant activity of a plant extract is to select the right method.
Antioxidant properties of the methanol extracts of the leaves and stems of celtis africana adeolu a. Inhibition data percentage inhibition were linearized against the concentrations of each extract and standard antioxidant. Variations in plant material, extraction method, processing and antioxidant assays employed might affect the concentrations of active compounds that could be reflected in the antioxidant activity. These compounds are widespread in the plant kingdom as part of our daily diet and are attractive as natural antioxidants.
Review on in vivo and in vitro methods evaluation of. The different extracts were dissolved in ethanol at a concentration of 50200 mgml. Antioxidant activity of methanol extracts of different. The ftc method was used to measure the amount of peroxide at the beginning of the lipid peroxidation, in which peroxide reacts with ferrous chloride and form ferric ion. Comparative study of antioxidant properties and total. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. Different parameters studied include phenolic contents, moisture, ash, crude fiber, fats and waxes. Antioxidant activity of methanol extracts of different parts of lantana.
In vitro antioxidant and anticancer activity of leea. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural systems, plant biotechnology and. Pdf different extracts from five medicinal plants in terms of phenolic compounds and. This is a typical problem in studies using plant extracts. Antioxidant and in vitro cytotoxic activity of extracts of. Antioxidantrich extracts of terminalia ferdinandiana.
Over the years, research on antioxidants and medicinal plants has gained enormous popularity and emerged as a potential therapeutic to prevent free radical generated damage in the human body. Tocopherol was used as a standard to evaluate the antioxidative activity of samples. Phytochemical screening and evaluation of antioxidant and. Antioxidant and antimicrobial properties of five medicinal. Issn total antioxidant capacity tac of fresh leaves of. The results showed that all the plant parts possessed antioxidant properties. Shanab2 1biochemistry department, faculty of agriculture, cairo university, giza, egypt.
In vitro nitric oxide scavenging activity of methanol. The reacted solution obtained 1 ml was used for tba assay. Abts radical cation was produced in the stable form using potassium persulphate. In the first thirtyfour pages, a total of three hundred and forty articles appeared and. Therefore, the assay for screening germplasm and hybrids should be.
Dpph free radical scavenging activity of the extracts of. Total phenolics content of leaf extract also highest 245. The present study evaluated the antioxidant effects of methanolic seed extract of a. Methanolic extracts of cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. The antioxidant assay of various extracts, using 2, 2diphenyl1picrylhydrazyl radical scavenging model, revealed that the ethanol and nbutanol extracts of d. The number of methods and variations in methods to measure antioxidants in botanicals that have been proposed has also increased considerably. The antioxidants present in the plant extracts react with oxidizing agents and their potential to reduce the oxidizing agents was evaluated spectrophotometrically 30. Research article open access antioxidant activity, total. Phytochemical screening and antioxidant activity of moroccan. Ferric reducing antioxidant power colorimetric assay protocol. The results from the antioxidant assay showed that extract of all plants can scavenge the radical to a certain extent. Leaf disc assays for rapid measurement of antioxidant.
Evaluation in any plant breeding program, however, has to deal with numerous plants, particularly at the early selection stage. Pdf evaluation of antioxidant activity of medicinal plant extracts. Medicinal plants used in the traditional medicine are wellknown significant sources of natural antioxidants. Antioxidant activities measured in methanol extract obtained using abts, dpph, frap, and orac assays from a single extract were measured three times to test the reproducibility of the assays. L with nanopure water or methanol in 96well plate, and.
The ethanol extract of the leaves exhibited the better antioxidant activity by dpph assay ic 50 1. It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds cook and samman, 1996. Dpph assay revealed that leaf extract had the highest antioxidant activity comparable with vitamin c leaf ic 50 16. Pdf methods for determining the antioxidant activity. Antioxidant activity by dpph assay of potential solutions. In vitro antioxidant activity of rubus ellipticus fruits. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. The powder samples and methanol extract of 11 medicinal plants were subjected to analysis of proximate composition and measurement of antioxidant activity. Original article comparison of abts, dpph, frap, and orac. Ic50 which is an inhibitory concentration of each extract required to reduce 50% of the nitric oxide. Among the extracts from curcuma longa, caffea arabica, tribulus terrestris, bacopa monnieri and trigonella foenum graecum, the curcuma longa and coffee bean extract. In vitro assays for the free radical scavenging capacity are usually based on the inactivation of radicals, such as hydroxyl oh and nitric oxide no radicals. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method.
There is a large variety of methods to determine this parameter, and the variability of experimental conditions found in the literature for each of the methods hinders such selection and the possibility of easily comparing the obtained results with those of other authors. The assays employed were ferric reducing antioxidant power, trolox equivalent antioxidant. Pdf the antioxidant potential of some herbal plant extracts commercial products was measured using various in vitro assays. Antioxidant capacity of selected plant extracts and their.
Plant material extracts were studied by in vitro methods, such as total phenolic. The dpph and frap assays showed no differences among determinations, while the abts and orac assays differed among runs table 2. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase. Fruit, vegetable and plant extractions can be done using acidmethanol for e. Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. The antioxidant activity of the aerial part extract of m. Antioxidant assay the antioxidant activity of the plant extracts was tested using two methods. For in vitro dpph, abts, and ppr antioxidant assays, 10. The determination of the total antioxidant activity frap assay in the extract is a modified method of benzie and strain 30.
Plants have a large number of bioactive compounds with high antioxidant activity. In this assay, a molecule or antioxidant with weak ah bonding will react with a stable free radical dpph 2,2diphenyl1picrylhydrazyl. Reviews of some of the methods have been published recently. Comparison of dpph and abts assays for determining.
Evaluation of antioxidant activity of some plant extracts. Total phenolic, total flavonoid, tannin content, and. Comparison of dpph and abts assays for determining antioxidant potential of water and methanol extracts of spirulina platensis emad a. However, use of bpe in antioxidant assays has shortcomings in that 1 bpe has lottolot variability in reactivity to peroxyl radicals, leading to inconsistency in assay results 15.1385 1488 269 1337 1522 707 1600 65 1597 67 538 1047 1347 128 1507 55 92 325 1136 447 903 361 1663 1356 936 969 740 1092 795 655 1224 1362 644 1247 1326